Table 1.

Tissue concentrations of ATV and RTV after acute single- and multiple-dose nanoART and native-drug administration at 10 mg/kg in mice (n = 5)

Tissue	Day of tissue collection	Concna
		ATV		RTV
		Native drug	NanoART	Native drug	NanoART
Liver	Day 1 after single dose	13.2 ± 4.2 ng/g	1,623.5 ± 1,377 ng/g	83.4 ± 15.2 ng/g	115.8 ± 19.2 ng/g
	Day 7 after two doses	1.4 ± 0.7 ng/g	21.7 ± 2.6 ng/g	1.3 ± 0.1 ng/g	40.2 ± 3.9 ng/g
	Day 14 after three doses	4.4 ± 1.6 ng/g	39.7 ± 11.2 ng/g	1.6 ± 0.4 ng/g	65.0 ± 8.5 ng/g
	Day 14 after single dose	1.3 ± 0.6 ng/g	7.4 ± 0.7 ng/g	1.6 ± 0.5 ng/g	14.2 ± 1.6 ng/g
Spleen	Day 1 after single dose	4.3 ± 2.1 ng/g	2,177.4 ± 1,482 ng/g	61.4 ± 19.2 ng/g	109.2 ± 30.5 ng/g
	Day 7 after two doses	*	2.7 ± 0.7 ng/g	*	35.2 ± 6.9 ng/g
	Day 14 after three doses	*	6.7 ± 3.7 ng/g	*	39.1 ± 4.4 ng/g
	Day 14 after single dose	*	1.3 ± 1.1 ng/g	*	11.7 ± 1.6 ng/g
Lung	Day 1 after single dose	2.2 ± 0.6 ng/g	345.7 ± 140.4 ng/g	23.9 ± 4.0 ng/g	20.2 ± 2.1 ng/g
	Day 7 after two doses	*	3.2 ± 0.4 ng/g	*	8.4 ± 1.3 ng/g
	Day 14 after three doses	*	5.2 ± 1.3 ng/g	*	13.6 ± 2.5 ng/g
	Day 14 after single dose	*	1.3 ± 0.6 ng/g	*	2.1 ± 0.3 ng/g
Kidney	Day 1 after single dose	4.5 ± 1.0 ng/g	101.1 ± 54.6 ng/g	54.6 ± 9.6 ng/g	75.6 ± 18.8 ng/g
	Day 7 after two doses	1.5 ± 0.2 ng/g	7.6 ± 2.0 ng/g	1.4 ± 0.2 ng/g	37.7 ± 2.9 ng/g
	Day 14 after three doses	1.3 ± 0.7 ng/g	5.8 ± 0.8 ng/g	2.0 ± 1.2 ng/g	52.1 ± 5.5 ng/g
	Day 14 after single dose	*	2.3 ± 0.6 ng/g	*	13.8 ± 3.2 ng/g
Brain	Day 1 after single dose	*	2.3 ± 0.5 ng/g	1.3 ± 0.3 ng/g	1.4 ± 0.2 ng/g
	Day 7 after two doses	*	*	*	*
	Day 14 after three doses	*	*	*	*
	Day 14 after single dose	*	*	*	*
Site of injection	Day 1 after single dose	87.9 ± 16.6 μg/g	1,121.6 ± 401 μg/g	48.0 ± 14.4 μg/g	984.4 ± 436 μg/g
	Day 7 after two doses	427.9 ± 135 μg/g	2,771.9 ± 429 μg/g	62.7 ± 26.8 μg/g	2,654.0 ± 417 μg/g
	Day 14 after three doses	42.3 ± 17.4 μg/g	1,968.0 ± 149 μg/g	0.6 ± 0.2 μg/g	1,514.3 ± 207 μg/g
	Day 14 after single dose	0.2 ± 0.2 μg/g	1,064.8 ± 134 μg/g	0.2 ± 0.1 μg/g	581.3 ± 94.6 μg/g

^aData represent means ± SEM. *, value is below the lower limit of quantitation (LLOQ).