Fig 1.

Comparative schematic presentation of the sequenced segments of the three novel erm(T)-carrying plasmids (pUR2941 [accession number HF583290], pUR1902 [HF583291], and pUR2940 [HF583292]) described in this study and the previously reported plasmids pSW49 (AM040730), pUB101 (AY373761), pKKS25 (FN390947), pNE131 (M12730), and pUR3912 (HE805623), as well as a chromosomal fragment of S. epidermidis ATCC 12228 (AE015929). The arrows indicate the extents and directions of transcription of antimicrobial [erm(T), tet(L), aadD, dfrK] and metal (cadD, cadX, copA, mco) resistance genes as well as genes involved in replication (rep49, repU, repL repA-like, putative rep-associated, putative rep, repA, and rep2), mobilization (mob), and others (ISLE49, smr, smr′, orf334). The 5′ and 3′ ends of the truncated rep49, repU, and repL genes and the 5′ end of the truncated mco gene in pUR2940 are likewise displayed. The different SSO regions detected are indicated as ssoA. The ISSau10 and IS431 copies are shown as black or gray boxes with a white arrow indicating the transposase gene tnp. The 8-bp direct target site duplications at the extremes of ISSau10 are shown in boxes and underlined. The 21-bp sequences of the integration site of the dfrK-carrying region are also boxed. The regions of >90% homology are shown in dark gray, while those between 80 and 90% similarity are displayed in light gray. The EcoRI (E) and BglII (B) cleavage sites are indicated. A size scale in kilobases is displayed in the lower right corner.