Fig 4.

Pfmspdbl2 DR allele parasites outcompete AC allele parasites in the presence of halofantrine. Parasites were mixed at equal ratios and grown with no drug or 1 nM halofantrine (HFN). Ratios of DR and AC parasites were determined after 1, 4, 8, and 14 asexual cycles by quantitative real-time PCR with TaqMan probes and are shown as fold abundance of the DR allele. Two biological replicates (#1 and #2) are shown, and error bars show standard deviations of technical replicates.