Fig 2.

MK6072 attenuated toxin B-mediated TNF-α expression in PBMCs. (A and B) PBMCs were incubated with no antibody (blank), control IgG, or MK6072 (30 μg/ml) for 30 min, followed by incubation with toxin B at 0 to 10 μg/ml for 4 h. (C and D) PBMCs were incubated with control IgG or MK6072 (0.1 to 50 μg/ml) for 30 min, followed by incubation with toxin B at 1 μg/ml for 4 h. Conditioned media were collected for TNF-α ELISA. TNF-α mRNA expression was detected by real-time RT-PCR. All experiments are representative of 3 independent experiments.