Fig 7.

MK3415 and MK6072 attenuated toxin A- and toxin B-mediated epithelial damage in human colonic explants. (A) Fresh human colonic explants were incubated with control IgG or MK3415 (30 μg/ml) for 30 min, followed by incubation with toxin A at 1 μg/ml for 16 h. (C) Explants were incubated with control IgG or MK6072 (30 μg/ml) for 30 min, followed by addition of toxin B at 10 μg/ml for 16 h. (B and D) Epithelial tissue damage was evaluated semiquantitatively as described in Materials and Methods. All experiments are representative of 5 patient samples.