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. 2013 Jul;57(7):3159–3167. doi: 10.1128/AAC.00811-12

Fig 3.

Fig 3

(A) Comparison by spot assays of the susceptibilities of S. cerevisiae strains BY4741 and BY4741_Δqdr2, harboring the cloning vector pGREG576 (v) or the CgQDR2 expression plasmid pGREG576_CgQDR2, to miconazole, tioconazole, and quinidine, at the indicated concentrations, in MM4 agar plates. The cell suspensions used to prepare the spots were 1:5 (b) and 1:25 (c) dilutions of the cell suspension used for column a. (B) Comparison of the susceptibilities of S. cerevisiae strains BY4741 (□, ◆) and BY4741_Δqdr2 (○, ▲), harboring the cloning vector pGREG576 (◆, ▲) or the CgQDR2 expression plasmid pGREG576_CgQDR2 (□, ○), under control conditions (A) and under stress induced by 7 mg/liter miconazole (B), 5 mg/liter tioconazole (C), or 4.5 mg/liter quinidine (D) in MM4 liquid medium. The inocula were prepared as described in Materials and Methods. Growth curves and spot assay images are representative of at least three independent experiments.