Fig 4.
(A) Time course accumulation ratio of [3H]clotrimazole in nonadapted cells of KUE100 (filled diamonds) or KUE100_Δcgqdr2 (filled squares) strains during cultivation in liquid BM in the presence of 30 mg/liter unlabeled clotrimazole. (B) Time course efflux ratio of [3H]clotrimazole in preloaded KUE100 (diamonds) or KUE100_Δcgqdr2 (squares) cells, upon a glucose pulse, in the presence (open symbols) or absence (filled symbols) of the protonophore CCCP, given after 30 min of passive accumulation of the radiolabeled drug. The accumulation ratio values are averages for at least three independent experiments. Error bars represent standard deviations.