Table 2.
Mutational spectra of gene knockout strains with enhanced evolutionary potentiala
| Mutation | Mutation rate/generation (10−8) |
|||||
|---|---|---|---|---|---|---|
| CC101 (A·T → C·G) | CC102 (G·C → A·T) | CC103 (G·C → C·G) | CC104 (G·C → T·A) | CC105 (A·T → T·A) | CC106 (A·T → G·C) | |
| None (WT) | —b | 1.6 | — | 2.3 | 0.4 | — |
| Δfur | — | 4 | — | 7.3 | — | 0.9 |
| ΔmiaA | — | — | 4.5 | 95.3 | 68.2 | — |
| ΔmutH | — | 88.3 | 1.5 | 6.7 | 19.5 | 82.1 |
| ΔmutL | — | 203.5 | 2.3 | 2.9 | 12 | 80.6 |
| ΔmutS | — | 18.2 | 2.6 | 28.6 | 0.5 | 312.7 |
a
Deletions from KEIO mutants were transferred into the WT (P90C, CGSC 8083) containing different types of lacZ mutations (CC101 to CC106, CGSC 8095 to CGSC 8100) by P1 transduction. Mutational frequencies were determined by lacZ reversion method. Mutation rates were calculated by the MSS maximum-likelihood method (see reference 29).
b
—, below detection limit (0.4/generation/108 cells).