Fig 2.

Confirmation of mechanism of action of aminothiazole series through siRNA knockdown of PI4KIIIβ. (A) Expression of PI4KIIIβ determined by qRT-PCR and Western blot analysis. The qRT-PCR results are averages of four samples with standard deviations (error bars). Two passages of H1-HeLa cells were examined by Western blotting (passage 6 and 44). The positions of molecular weight in kDa are shown to the right of the Western blot. (B) Effect of knockdown of various lipid kinases or irrelevant gene (IRR) via siRNA transfection on HRV infection of H1-HeLa cells at two multiplicities of infection (MOIs). Data are averages of four samples with standard deviations. The data are representative of three experiments. (C) Expression of lipid kinases as determined by qRT-PCR after siRNA treatment for 6 days (averages of four samples with standard deviations). (D) Effect of compound 1 targeting PI4KIIIβ and the protease inhibitor rupintrivir on HRV infection in siRNA-transfected H1-HeLa cells (MOI of 0.05). Data are averages of four samples with standard deviations. The data are representative of three experiments.