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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Apr 4;1830(8):4040–4045. doi: 10.1016/j.bbagen.2013.03.028

Fig. 4.

Fig. 4

Effect of proteasome inhibitors on PKCη downregulation. A, MCF-7 cells were pretreated with 10 μM MG-132 for 30 min, followed by treatment with 1 μM Gö 6983 or 25 μM Ly294002 for 12 h. Western blot analysis was performed with total cellular extracts using the indicated antibodies. GAPDH was used as a loading control. B, Densitometric quantification of PKCη protein levels from 3 separate experiments corrected for loading. Data represents the mean +/- s.e.m. The asterisk (*) indicates significant difference of MG-132 treated cells from control or treatment with the inhibitors alone using Student's t-test. *, P<0.05; **, P<0.005 C, T47D cells were pretreated with 10 M MG-132 or 10 μM lactacystin for 30 min, followed by treatment with 1 μM Gö 6983 or 10 μM Ly294002 for 15 h. Western blot analysis was performed with total cellular extracts using the indicated antibodies. Actin was used as a loading control.