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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Apr 4;1830(8):4040–4045. doi: 10.1016/j.bbagen.2013.03.028

Fig. 5.

Fig. 5

Effect of PDK1 and PKCε on PKCη downregulation. A, MCF-7 cells were transfected with control non-targeting siRNA, PDK1 siRNA or were left non-transfected (NT). Cells were then pre-treated with either 10 μM MG-132 or 10 μM lactacystin, followed by treatment with 25 μM Ly294002. Western blot analysis was performed with indicated antibodies. Actin was used as a loading control. B, T47D cells were infected with adenovirus vector containing GFP or PKCε construct and then treated with 1 μM Gö 6983 for 15 h. Western blot analysis was carried out with indicated antibodies.