Fig 2.
Size exclusion chromatography reveals aggregation of the soluble multimers. After Ni2+-chromatography, the purified TrxL2 proteins were analyzed via size exclusion chromatography using a Superdex 200 column. Except for the monomeric TrxL2, large portions of the multimerized antigens were present as soluble high-molecular-weight forms with an average of ∼600 kDa. The aggregate (∼600 kDa) and nonaggregate elution peaks are labeled, and the elution volumes are indicated above the peak. mAU, milli-absorbance units.