Fig 4.

Aggregate and nonaggregate forms of tetrameric TrxL2 are equally immunogenic. The HPV-16 neutralization titers for the 4×-TrxL2, 4×-TrxL2 nonaggregate (non-agg), and/or aggregate (agg) immune sera are shown. Serum samples were serially diluted 1:3 starting with a 1:100 dilution. Each dot represents one mouse serum sample, with horizontal bars indicating the mean titers. Animals were immunized three times at biweekly intervals with 25 μg of antigen formulated with 50% (vol/vol) Montanide ISA 720. Final serum samples were collected 8 weeks after the third immunization.