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Splenic bacterial burden 24 h after challenge. Mice were immunized as indicated on the x axis, challenged with 6 × 104 CFU of B. pseudomallei K96243, and culled after 24 h, and the splenic bacterial burden was assessed. Each point represents a mouse. Significance levels are indicated. The limit of detection (LoD) was ∼10 CFU per spleen.
