Fig 2.

Detection of anti-K99, anti-STa, and anti-BVDV E2 antibodies from mouse serum and fecal samples. Anti-K99, anti-STa, and anti-BVDV IgG (top panels) and IgA (bottom panels) antibodies were titrated from serum and fecal suspension samples of the immunized mice (solid diamonds) and control mice (blank squares). Five hundred nanograms of heat-extracted K99 fimbriae, 10 ng of STa-ovalbumin conjugates, and 500 ng of MBP-E2 fusion proteins were used to coat each well in an ELISA to titrate anti-K99, anti-STa, and anti-BVDV E2 antibodies, respectively. HRP-conjugated goat anti-mouse IgG (1:3,000) and IgA (1:1,000) were used as the secondary antibodies. Optical densities of greater than 0.3 (after subtracting the background reading) from the greatest dilution were used to calculate antibody titers (in log₁₀), or OD values less than 0.3 after subtraction of the background were directly compared between groups. Bars are means and standard errors.