Fig 3.

Antibody neutralization against STa toxin and adherence inhibition against K99 fimbrial E. coli bacteria. (A) Antibody neutralization against STa toxin using T-84 cells and an EIA cGMP kit. Serum samples of immunized mice prevented STa toxin from stimulating intracellular cGMP in T-84 cells. (B) Antibody inhibiting adherence of K99 fimbrial E. coli I297 to INT-407 or IPEC-J1 cells. INT-407 and IPEC-J1 cells had significantly fewer I297 bacteria adhered when pretreated with the serum samples from the immunized mice. Bars are means and standard errors.