Table 1.
Bacterial and viral strains and plasmids used in this study
| Strain or plasmid | Relevant property(ies)a | Reference |
|---|---|---|
| E. coli strains | ||
| I297 | Recombinant E. coli strain expressing K99 fimbria | 29 |
| BL21 | B F− ompT hsdS(rB− mB−) gal dcm | GE Healthcare |
| 9196 | BL21/pFanC-STa-E2 | This study |
| 9210 | BL21/pMAL-E2 | This study |
| BVDV strain | ||
| NADL | Bovine viral diarrhea virus 1; NADL strain | ATCC VR-534 |
| Plasmids | ||
| pK99 | pBR322 with a cloned 7.1-kb K99 BamHI fragment | 29 |
| pET28a | Expression vector, His-tagged, T7, lacI, Kanr | Novagen |
| pMAL-p5X | Expression vector with MBP, tac, IacIq, Ampr | New England Biolabs |
| pFanC-STa-E2 | pET28a with a cloned FanC-STa-E2 chimeric fusion | This study |
| pMAL-E2 | pMAL-p5X with a cloned BVDV E2 gene | This study |
a
Kanr, kanamycin resistant; Ampr, ampicillin resistant.