Table 2.
PCR primers designed to amplify the fanC gene and to construct the chimeric FanC-STa-E2 genea
| Primer | Nucleotide sequence (5′–3′) |
|---|---|
| FanCNheI-F2 | ATGATGGCTAGCACACTCCTAGCTATTATCTTAGGT |
| FanCEagI-R | TCATCGATACGGCCGCAATGTAA |
| STa12/FanC91-F | GAACTTTGTTGTAATTTTGCCTGTGCTGGATGTGGAAATACTGCTGCTAAAGGATACCAT |
| STa12/FanC107-R | GGCAAAATTACAACAAAGTTCACAGCAGTAAAATGTGTTGTTAGACCAGTCAATACGAGC |
| BVDV/FanC116-F | GCCTTGCCGACCAGTGTGGTATTCGCTAATATTAATACTTCATTCACTACG |
| BVDV/FanC126-R | ACTGGTCGGCAAGGCTCTTGTATGCAAAGTCATATGGTATCCTTTAGCAGC |
| BVDV/FanC154-F | CTACTATACAAAGGGGGCTCTGGTGGATATAAAGCTGGCGTATT |
| BVDV/FanC162-R | GCCCCCTTTGTATAGTAGTTGGTCCAGCTGGGCTGAATAGTTAAATGACT |
| BVD-E2Nde1-F* | ATTTCACATATGCACTTGGATTGAAAACCTGAA |
| BVD-E2BamH1-R* | TCTGTAGGATCCAGGCATAGGTCCGAGTTTGGT |
a
PCR primers marked with an asterisk were used to produce the MBP-E2 chimeric gene for the recombinant MBP-E2 protein as coating antigens. Underlined nucleotides indicate restriction sites, and italicized nucleotides are of the inserted STaP12F or E2 epitope.