Fig 2.
Ypt52p and Ypt53p can suppress vps21Δ/Δ mutant hyphal growth and stress defects. A wild-type allele of either VPS21 or YPT52 or an ACT1p-YPT53 construct was introduced into the vps21Δ/Δ mutant. Similar constructs encoding putatively inactive (YPT52S26N and YPT53T27N) and active (YPT52Q73L and YPT53Q75L) conformational mutant alleles of either gene, were also introduced into the vps21Δ/Δ mutant strain (A and B only). (A and B) Cell suspensions of each strain were applied as spots to 10% FBS agar and incubated at 37°C for 4 days. Similar results were found after incubation on M199 agar at 37°C (see Fig. S3A in the supplemental material). (C and D) Cell suspensions of each strain were prepared by serial dilution, applied to YPD agar or to YPD agar supplemented with 25 μg/ml Congo red or 0.05% SDS, and incubated at 30°C for 2 days.