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Loss of AP-3 trafficking dramatically increases vacuolar size in the endosome-deficient vps21Δ/Δ ypt52Δ/Δ mutant yeast but exacerbates the hyphal growth defects. Vacuole morphology was determined by CMAC staining of yeast cells grown in YPD at 30°C (top panels) or hyphal cells grown in M199 medium at 37°C (bottom panels). Hyphal growth on 10% FBS agar was also compared (center panels).
