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Localization of conventional kinesin, NKIN, and NKIN3 (kinesin-3) and the corresponding rigor versions. (A) GFP-NKIN localizes in the cytoplasm but not in nuclei (dark spots). (B) Several microtubules are decorated by GFP-NKINrigor, most prominently at the hyphal tip. (C) GFP-NKIN3 localizes throughout the whole hypha. (D) The rigor version of NKIN3 does not bind to microtubules. Scale bar, 10 μm.
