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Colocalization of GFP-YPT52- and FM4-64-labeled putative endosomes. (A and B) Most of the FM4-64-stained vesicles colocalize with the GFP-YPT52-labeled spot in a young hypha (A) as well as in leading hyphae (B) (arrowheads). A total of 31% of the spots do not colocalize (asterisks). DIC, differential interference contrast. Scale bars, 5 μm.
