Fig 2.

UPR genes are induced by multiple ER stress agents. Freshly purified tachyzoites were exposed to 10 μM tunicamycin (TUN), 5 μM A23187, or vehicle (DMSO) for 1 h. Levels for two UPR gene transcripts, a calreticulin family domain member (TGME49_077230) and a putative E3 ubiquitin ligase (TGME49_095670), were measured by qPCR. The histograms represent statistically significant changes (P < 0.01) between ER-stressed samples and the DMSO control, with standard errors (SE) indicated by the error bars.