Fig 4.

Polysome association of Toxoplasma gene transcripts is suggested to be reduced in response to ER stress. (A) Histogram representing the number of different gene transcripts (y axis) associated with polysomes (% polysomal RNA) from parasites cultured in the presence of tunicamycin (TUN) or vehicle (black and white bars, respectively). The arrow denotes a subset of mRNAs that are poorly translated in the absence of stress. (B) The percentage of polysomal RNA of the preferentially translated (i.e., enhanced) transcripts (following exposure to tunicamycin) represented in a histogram in the presence of tunicamycin or vehicle (black and white bars, respectively).