Table 2.

Tunicamycin induces expression of genes consistent with the unfolded protein response in other eukaryotes^a

ToxoDB Version 5 ID	Product	Fold induction	P value	TM (no.)	SP
Protein modification
TGME49_007070	Glycosyl transferase	4.10	0.007	No	No
TGME49_097720	Glycosyl transferase domain	6.84	0.044	No	No
Protein degradation
TGME49_017160	Derlin-1	6.97	0.012	4	Yes
TGME49_095670	E3 ubiquitin ligase	2.80	0.010	No	No
TGME49_071490	Endoprotease	7.72	0.005	1	Yes
Quality control and protein folding
TGME49_063170	Heat shock protein	9.36	0.000	No	No
TGME49_077230	Calruticulin	3.44	0.002	No	No
TGME49_094870	Universal stress protein/chaperone	4.05	0.007	No	No
Vesicle transport
TGME49_073070	GTPase activating protein for Arf domain-containing protein	3.55	0.044	No	Yes
TGME49_014170	Prenyltransferase	4.14	0.041	No	Yes
Lipid and membrane metabolism
TGME49_063740	ATP-binding cassette, lipid transporter	5.31	0.005	5	No
TGME49_078110	1,3-Beta-glucan synthase	4.34	0.038	11	No
TGME49_046010	Esterase/lipase	10.76	0.008	No	No
TGME49_050360	Esterase/lipase	1.42	0.043	No	No

^aShown are Toxoplasma genes whose expression is induced by tunicamycin treatment. Like other eukaryotes, the Toxoplasma UPR contains factors involved in protein modification, folding, degradation, vesicle transport, and lipid/membrane metabolism. Statistical analysis was performed as described for Table 1. TMHMM and SignalP were used to predict the presence or absence of transmembrane (TM) domains and signal peptide (SP) sequences.