Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cell. 2012 Sep 20;151(1):194–205. doi: 10.1016/j.cell.2012.09.001

Figure 1. DNA methylation and small RNA accumulation during pollen development.

Figure 1

(A) Pollen development: the uninucleate microspore divides asymmetrically giving rise to bicellular pollen, which consists of a larger vegetative cell embedding a smaller generative cell. A second mitotic division of the generative cell originates two sperm cells. The three cell types analyzed in this study were stained with DAPI to highlight heterochromatin, which is lost in the vegetative nucleus (VN) but not in the sperm cell nuclei (SC) (bar = 10μm). (B) Heat map representation of DNA methylation. Bisulfite sequencing of genomic DNA from each cell type was performed as described. Methylation density is represented in 10kb blocks, separated by context and cell type. CG (CG methylation), CHG (CHG methylation), CHH (CHH methylation), INF (Inflorescence), MS (Microspore), VN (Vegetative nucleus), SC (Sperm Cell), EMB (Embryo). The maximum value of the heat map is calibrated to the VN. The outer annotation track highlights the position of transposons (TEs).