TABLE 3.
PHB, CO2, and methyl ketone production in R. eutropha strains bearing the methyl ketone pathway under induced and uninduced heterotrophic conditions (RAMOS experiments)
| Conditions and straina | Carbon distribution (% of fructose C consumed)b |
||
|---|---|---|---|
| PHB | CO2 | MK | |
| Induced conditions | |||
| Δbeta ox (JM13) | 4 ± 0.73 | 0.1 | 2.2 ± 0.05 |
| Δbeta ox ΔphaCAB (JM15) | 0.3 ± 0.03 | 0.5 | 3.5 ± 0.66 |
| Uninduced conditions | |||
| Δbeta ox | 11 ± 0.77 | 0 | 0 |
| Δbeta ox ΔphaCAB | 0.1 | 0 | 0 |
a
All strains carried the MK pathway (Table 1).
b
Data are averages for two independent cultures ± standard deviations (standard deviations were not calculated for values near the detection limit).