Fig 5.

Effects of disruption of lpdB, lpdC, and lpdD on gallate and protocatechuate decarboxylation in L. plantarum WCFS1. HPLC chromatograms of L. plantarum cultures incubated in 3 mM gallic acid (A) or protocatechuic acid (B) are shown for L. plantarum WCFS1 (wild type [wt]), L. plantarum WCFS1(pUCE191-lpdB) (lpdB mutant), L. plantarum WCFS1(pUCE191-lpdC) (lpdC mutant), and L. plantarum WCFS1(pUCE191-lpdD) (lpdD mutant). Results for uninoculated medium are also shown (control). The gallic acid (GA), protocatechuic acid (PA), pyrogallol (P), and catechol (C) detected are indicated. Chromatograms were recorded at 280 nm.