Table 1.
Results of direct PCR performed on fecal samples collected from wood bison herds in northern Canada, using three separate genetic targets for M. avium subsp. paratuberculosis
| Wood bison herd | Sample size (estimated herd size) | No. (%) positive fora: |
|||
|---|---|---|---|---|---|
| IS900 | TMRb | F57 | TMRb + F57 | ||
| Elk Island National Park | 31 (361) | 10 (32) | 4 (40) | 2 (20) | 2 (20) |
| Wood Buffalo National Parkc | 23 (4,958) | 11 (48) | 6 (55) | 1 (9) | 2 (18) |
| Aishihikc | 31 (1,230) | 8 (26) | 3 (38) | 0 | 3 (38) |
| Nordquist | 10 (155) | 3 (30) | 3 (100) | 0 | 0 |
| Etthithun | 14 (226) | 7 (50) | 5 (71) | 0 | 0 |
| Nahannic | 48 (400) | 16 (33) | 10 (63) | 0 | 3 (19) |
| Mackenziec | 51 (1,438) | 8 (16) | 4 (50) | 0 | 2 (25) |
| Grande Detourc | 45 (1,790)d | 12 (27) | 9 (75) | 1 (8) | 1 (8) |
| Hay Zama | 14 (524) | 5 (36) | 3 (60) | 0 | 0 |
| Total | 267 | 80 (30) | 47 (59) | 4 (5) | 13 (16) |
a
Only IS900-positive samples were tested for the other two targets.
b
TaqMan MAP reagents (Applied Biosystems), a commercially available PCR kit that targets ISMAP02.
c
IS1311 restriction enzyme analysis performed on samples from this herd.
d
Estimate is for Slave River Lowlands, which includes Grande Detour and Hook Lake populations.