Fig 1.

Growth profiles of M. barkeri incubated at 37°C and 15°C. Optical density (at 600 nm) measurements (A) and percent CH₄ headspace measurements (B) were determined in tandem for M. barkeri grown at 37°C with H₂/CO₂ plus methanol ■, methanol alone ●, and H₂/CO₂ alone ▲ and at 15°C with H₂/CO₂ plus methanol □, methanol alone ○, and H₂/CO₂ alone △. Error bars indicate the standard deviations and are the result of at least three replicates. Data represent phase 1 (samples I and II), phase 2 (sample III), and phase 3 (sample IV) of the LTAS experiment.