Table 2.
Complementation of ATCC 14479-derived malonate mutants with different complementing plasmids
| Complementing plasmid | Growth of the following mutant with the indicated complementing plasmida: |
||
|---|---|---|---|
| matA mutant (LMB134) | matB mutant (LMB510) | matC mutant (LMB136) | |
| pLMB605 (pmatA) | − | − | − |
| pLMB610 (pmatAB) | − | − | ND |
| pLMB606 (pmatC) | ND | ND | − |
| pLMB628 (pmatABC) | − | − | − |
| pLMB654 (pmatRABC) | + | + | + |
| pLMB509 (pTau vector)b | ND | ND | − |
| pLMB653 (pTau::matC)b | ND | ND | + |
a
Growth of mutant strains derived from R. leguminosarum ATCC 14479 is scored as shown in Fig. S1 in the supplemental material: −, no growth; +, poor growth; ND, not determined. Growth was measured on UMS agar (see detailed methods in the supplemental material) supplemented with 5 mM malonate as the sole carbon source.
b
Grown on 5 mM malonate plus 0.1 mM taurine.