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Expression of nisin measured in terms of activity. In the control strain, NZ9800(pNZ-nisA) (20), nisA and the modification enzymes that process nisin are controlled by PnisA. In NZ9800(pCZ-nisA), nisA was controlled by PczcD. A constant concentration of nisin (5 ng/ml) was used to induce both strains. In NZ9800(pCZ-nisA), no activity was detected in the absence of Zn2+, whereas increasing amounts of Zn2+ led to the production of nisin proportionally to the concentration used.
