Fig 4.
Western blot analysis of germination proteins in total lysates of spores of various B. subtilis strains. Total spore lysates from spores of B. subtilis strains PS533 (wild type) (lanes 1 and 5), PS4256 (ΔgerKDbs) (lanes 2 and 6), PS4213 (amyE::gerKDbs ΔgerKDbs) (lanes 3 and 7), and PS4314 (amyE::PsspB-gerKDbs) (lanes 4 and 8) were isolated, and samples with identical amounts of protein from ∼7 × 106 spores (lanes 1 to 4) or 1.8 × 106 spores (lanes 5 to 8) (with amounts of protein adjusted appropriately based on preliminary SDS-PAGE and Coomassie blue staining) were run on SDS-polyacrylamide gels and subjected to Western blot analysis with antisera against various germination proteins as described in Materials and Methods. The strips probed for the various antigens were from several different blots.