Fig 3.
The residue at position 113 affects the copper response in GolS. β-Galactosidase activity from a golB::lacZ transcriptional fusion expressed by cells carrying either golS (GolS) or the mutant alleles coding for GolSL, GolSA113P, GolSA116D, GolSL117S, or GolSP118A, in an otherwise wild-type genetic background (A and C) or in a Δ(cueR-copA) genetic background (B and D). Cell cultures were grown overnight in SM9 containing the indicated concentrations of AuHCl4 (A and B) or CuSO4 (C and D). The data correspond to mean values of four independent experiments performed in duplicate. Error bars correspond to the standard deviations.