Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotypea | Source, derivation, or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F− ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen |
| χ7213 | thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 ΔasdA4 Δ(zhf-2::Tn10) thi-1 RP4-2-Tc::Mu [λpir] | 54 |
| S. Typhi | ||
| χ3769 (Ty2) | Wild-type; cys trp rpoS | 55 |
| χ8444 | ΔphoPQ23 | 56 |
| χ11118 | ΔPfur81::TT araC ParaBAD fur | Ty2 |
| χ11500 | Δ(adiA-adiC)-4806 | Ty2 |
| χ11548 | ΔaroD1299 | Ty2 |
| χ11552 | ΔaroD1299 ΔPadiA203::TT araC ParaBAD adiA | χ11548 |
| χ11564 | ΔaroD1299 ΔPadiA276::TT rhaSR PrhaBAD adiA | χ11548 |
| χ11568 | ΔaroD1299 ΔPadiA276::TT rhaSR PrhaBAD adiA Δ(PadiY-adiY-PadiC)-119 adiC | χ11564 |
| χ11622 | ΔphoPQ23 ΔPadiA276::TT rhaSR PrhaBAD adiA Δ(PadiY-adiY-PadiC)-119 adiC | χ8444 |
| χ11623 | ΔPfur81::TT araC ParaBAD fur ΔPadiA276::TT rhaSR PrhaBAD adiA Δ(PadiY-adiY-PadiC)-119 adiC | χ11118 |
| χ11636 | ΔaroD1299 Δ(PadiY-adiY-PadiC)-119 adiC | χ11548 |
| Shigella flexneri 2457T | S. flexneri 2a, wild type; Pcr+ Mal− λr | 57 |
| Plasmids | ||
| pET28a | Protein synthesis vector, T7 promoter; Kanr | Novagen |
| pYA3700 | Vector containing tightly regulated TT araC ParaBAD cassette | 58, 59 |
| pYA4181 | Suicide vector to generate ΔPfur81::TT araC ParaBAD fur mutation | 35 |
| pYA4278 | Suicide vector; sacB mobRP4 oriR6K Cmr | 60 |
| pYA4895 | Suicide vector to generate ΔaroD1299 mutation | pYA4278 |
| pYA5066 | Suicide vector to generate Δ(adiA-adiC)-4806 mutation | pYA4278 |
| pYA5072 | Suicide vector to generate Δ(PadiY-adiY-PadiC)-119 adiC mutation | pYA4278 |
| pYA5075 | Intermediate vector for creation of ΔPadiA203::TT araC ParaBAD adiA | pYA3700 |
| pYA5081 | Suicide vector specifying tightly regulated rhaSR PrhaBAD cassette | 61 |
| pYA5085 | Protein synthesis vector with N-terminal His tag on AdiA | pET28a |
| pYA5089 | Suicide vector to generate ΔPadiA203::TT araC ParaBAD adiA mutation | pYA4278, pYA5075 |
| pYA5093 | Suicide vector to generate ΔPadiA276::TT rhaSR PrhaBAD adiA mutation | pYA5089, pYA5081 |
a
In genotype descriptions, the subscripted designation number refers to a composite deletion and insertion of the indicated gene. P, promoter; TT, T4 ip III transcription terminator; Cmr, chloramphenicol resistance; Kanr, kanamycin resistance.