Table 1.
E. coli strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source |
|---|---|---|
| Strains | ||
| MC4100 | F− araD139 (argF-lac) U169 rpsL150 relA1 flbB5301 deoC ptsF25 rbsR | 29 |
| BW25113 | K-12 wild type | 30 |
| JW5713 | BW25113 ΔubiC::Kanr | 30 |
| ECL5001 | MC4100 λΦ(cydA′-lacZ) | 7 |
| ECL5039 | ECL5001 ΔubiCA::Kanr | 23 |
| ECL5003 | MC4100 Δfnr::Tn9(Chlr) λΦ(cydA′-lacZ) | 7 |
| IFC5006 | ECL5003 ΔmenFDHB::Kanr | This work |
| ECL594 | MC4100 sdh+ Φ(sdh-lacZ) arcBl zgi::Tn10 | 28 |
| IFC5007 | MC4100 Φ(sdh-lacZ) from ECL594 | This work |
| IFC5008 | IFC5007 ΔubiCA::Kanr from ECL5039 | This work |
| IFC5009 | ECL5001 ΔubiC::Kanr from JW5713 | This work |
| Plasmids | ||
| pEXT21 | Low-copy-no. vector; Sptr | 32 |
| pBR322 | Cloning vector; Ampr Tetr | 31 |
| pMX517 | Full-length arcB under the control of the ara promoter; Ampr | 9 |
| pQE30ArcB78-778 | ArcB78-778 in pQE30 under an IPTG-inducible promoter | 15 |
| pMX537 | pEXT21::ubiCA; Sptr | This work |
| pMX538 | pBR322::menFDHBCE; Ampr | This work |
a
Kanr, kanamycin resistant; Chl, chloramphenicol; Spt, spectinomycin; Amp, ampicillin; Tet, tetracycline.