Table 2.
DNA primers used in this work
| Name | Sequence (5′–3′)a | Use |
|---|---|---|
| menF-up-pKD-Fw | CCCCGTATAATGTGAGGCTTTTAACAGGGAGAGGTCCGCGTGTAGGCTGGAGCTGCTTC | Forward primer for men operon deletion |
| menB-down-pKD-Rv | CGTCCATGGGGATCTGCCAGCGGTATACCTGCGCGCTACGCATATGAATATCCTCCTTAG | Reverse primer for men operon deletion |
| menF-Fw | CCCGGATCCGTGATACACGTATCGATG (BamHI) | Forward primer for men operon cloning and men mutant confirmation |
| menE-Rv | CCCAAGCTTGACCAGCCATTCCATTGC (HindIII) | Reverse primer for men operon cloning and men mutant confirmation |
| ubiC-Fw | GATACCCAACAGATGATCG | Forward primer for ubi operon cloning and ubi mutant confirmation |
| ubiA-Rv | CCCAAGCTTCGCACGCTACAGCTGC (HindIII) | Reverse primer for ubi operon cloning and ubi mutant confirmation |
| k1-pKD4 | GTCATAGCCGAATAGCC | Primer for mutant genotype confirmation |
| k2-pKD4 | GTGCCCTGAATGAACTG | Primer for mutant genotype confirmation |
a
Restriction enzymes whose sites were introduced for subsequent cloning (underlined) are indicated in parentheses.