Table 2.
Primer | Sequence (5′–3′)a |
---|---|
OEF212 | CTCTTCTGCCGATGAAGTTTCTGG |
OEF262 | TTTCCAGAGGATCATTCGCCGTTCAATTTCTTCACC |
OEF264 | TCTCGAGGTATTGAACTCCTTCCAC |
OEF273 | AAGGATCCTGACGGAAGAAGATATTCGC |
OEF274 | TGGCATGCGACAAATTCTCAATTATGCC |
OEF279 | TCCCATGGAACAAAAAGAATATATGGAATATCGG |
OEF280 | TTAGGATCCGTTTTCTTTTATTTCTTTTAAAC |
OEF281 | CCTGCAGACAAGAAAACGGTGCTC |
OEF324 | CGAATGATCCTCTGGAAA |
OEF333 | TTCACCTAGCGGCCGATATTC |
OEF339 | GCAACGAAATGGTGGAACAG |
OEF340 | AAGGCATCGGCAATCTCTAAG |
OEF353 | FAM-AGGCATAATTGAGAATTTGTC |
OEF354 | FAM-GGAGGATGCGATTGTTTCG |
OEF355 | FAM-ATTAATTTGGACAGTTTGTCG |
OEF501 | TTGTCGACCATAAACTTCACCTCCTG |
OEF502 | AAGGATCCGGGAGGTTCTGGCAAAG |
OEF504 | AAGCATGCCGTTTCCAACTTCGTTAC |
OEF594 | FAM-CGAATGATCCTCTGGAAA |
OEF651 | FAM-GCCGCTAGGTGAAGAAATTG |
OEF652 | FAM-CGATAATCTGCAACGATTCG |
OEF658 | GATGCCACAAAAGGACTGTTTGA |
OEF659 | CAGGTTGGACGACTGTTCCA |
OEF689 | AAGCATGCGGCATAATTGAGAATTTGTC |
OEF690 | AAGCATGCTTAATTTGGACAGTTTGTCG |
OEF694 | AAGCATGCGAGTGTGTTGATAGTGC |
OEF695 | TTTTCTTAAGCTCCTTTCGCTTTCTTC |
FR.lac5 | ATGGGATAGGTCACGTTGGTGTAGAT |
Underlined nucleotides specify the restriction enzyme site used for cloning. FAM indicates coupling with 6-carboxyfluorescein.