Fig 1.

Genetic map and origin of transfer (oriT) of plasmid pMV158. (A) Scheme of pMV158. Arrowheads indicate the direction of transcription of the four protein-coding genes (mobM, copG, repB, and tetL). The positions of the oriT and the origins of the leading-strand (dso) and lagging-strand (ssoU and ssoA) syntheses are shown. (B) DNA sequence of the oriT of pMV158 (coordinates 3564 to 3605). The three identified inverted repeats (IR1, IR2, and IR3) are indicated by arrows, and the nick site is indicated by a vertical arrowhead. The oligonucleotides used in this study are also depicted (Table 1).