Table 2.
Protein | No. of residuesa | Calculated massb (Da) | Measured massc (Da) | Activity on DNA |
|
---|---|---|---|---|---|
Supercoiled | Single stranded | ||||
MobM | 493 | 57,692.3 | ND | Yes | Yes |
MobMN199 | 198 | 23,115.2 | 23,195.3d | Yes | No |
MobMN243 | 242 | 28,393.6 | 28,409.7e | Yes | Yes |
Number of total amino acid residues, excluding Met1, which is processed in the expression systems used in this study.
Monoisotopic, excluding Met1 (see footnote a).
Masses as determined by ESI-MS (see the text).
Mass corresponds to MobMN199 with one phosphorylated residue.
Mass corresponds to MobMN243 oxidized at one position.