Table 2.
Characteristics of 34 conjugation-positive E. coli isolates carrying blaCTX-M-14 genes described in the studya
| Isolate | Farm | Origin | Etest MIC (mg/liter) |
Other β-lactamase | Addiction system(s)b | Plasmid Inc type | Non-β-lactam resistance pattern | |||
|---|---|---|---|---|---|---|---|---|---|---|
| CT | CTL | TZ | TZL | |||||||
| M9-7 | L | Cattle feces | >16 | 0.023 | ≤0.5 | ≤0.064 | TEM-1 | ccdAB, pndCA, srnBC | I1-Iγ | GEN STR |
| M9-8 | L | Calf feces | >16 | 0.032 | ≤0.5 | 0.064 | TEM-1 | ccdAB | NT | GEN STR |
| M9-9 | L | Calf feces | 16 | 0.032 | ≤0.5 | 0.064 | TEM-1 | srnBC | NT | GEN STR |
| M9-10 | L | Calf feces | >16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | pndCA, srnBC | NT | GEN STR SXT TET |
| M9-13 | M | Calf feces | >16 | 0.023 | ≤0.5 | 0.094 | hok-sok, pndAC, srnBC | F | NEO STR TET | |
| M9-14 | M | Calf feces | >16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | ccdAB, vagCD, srnBC | F | NEO NAL SXT TET |
| M9-15 | N | Calf feces | 16 | 0.047 | ≤0.5 | 0.125 | pndCA | I1-Iγ | APR CIP NEO NAL STR SXT TET | |
| M9-16 | N | Calf feces | >16 | 0.023 | ≤0.5 | 0.094 | TEM-1 | ccdAB, vagCD, srnBC | NT | CHL CIP GEN NEO NAL STR SXT TET |
| M9-17 | L | Feed | >16 | 0.032 | ≤0.5 | 0.064 | TEM-1 | pndCA, srnBC | NT | NEO STR TET |
| M9-18 | L | Ground floor | 12 | 0.032 | 0.5 | 0.094 | TEM-1 | ccdAB, srnBC | NT | NEO NAL STR SXT TET |
| M9-19 | L | Ground fence | 12 | 0.032 | ≤0.5 | 0.064 | TEM-1 | pndCA, srnBC | NT | APR CIP NEO NAL STR SXT TET |
| M9-20 | L | Parlor fence | 16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | pndCA, srnBC | NT | NEO STR TET |
| M9-21 | L | Parlor floor | >16 | 0.032 | ≤0.5 | 0.064 | pndAC, srnBC | NT | NEO NAL STR SXT TET | |
| M9-22 | L | Milking cup | >16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | ccdAB, pndAC, srnBC | NT | APR CIP NEO NAL STR SXT TET |
| M9-23 | L | Teat | 16 | 0.032 | 0.5 | 0.064 | TEM-1 | pndAC, srnBC | NT | NEO STR TET |
| M9-24 | L | Teat | >16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | ccdAB, pndAC, srnBC | NT | NEO NAL STR SXT TET |
| M9-25 | L | Teat | 8 | 0.023 | ≤0.5 | 0.064 | TEM-1 | pndAC, srnBC | NT | APR CIP NEO NAL STR SXT TET |
| M9-26 | L | Teat | 16 | 0.032 | ≤0.5 | 0.064 | TEM-1 | pndAC, srnBC | NT | NEO NAL STR SXT TET |
| M9-27 | L | Teat | 16 | 0.016 | ≤0.5 | ≤0.064 | TEM-1 | ccdAB, srnBC | NT | NEO NAL STR SXT TET |
| M9-28 | L | Teat | 16 | 0.032 | ≤0.5 | 0.094 | TEM-1 | ccdAB, srnBC | NT | APR CIP NEO NAL STR SXT TET |
| M9-29 | L | Teat | 8 | 0.032 | 0.5 | 0.094 | TEM-1 | ccdAB, srnBC | NT | NEO NAL STR TET |
| M9-30 | L | Teat | 16 | 0.023 | ≤0.5 | 0.064 | TEM-1 | pndAC, srnBC | NT | NEO NAL STR SXT TET |
| M9-31 | L | Teat | 12 | 0.023 | ≤0.5 | 0.064 | TEM-1 | ccdAB, srnBC | NT | APR CIP, NEO NAL STR SXT TET |
| M9-32 | L | Farmer's hand | 12 | 0.023 | ≤0.5 | 0.064 | TEM-1 | pndAC, srnBC | NT | NEO STR TET |
| M9-33 | M | Ventilation | >16 | 0.032 | 0.5 | 0.094 | TEM-1 | ccdAB, vagCD, srnBC | F | NEO NAL STR SXT TET |
| M9-34 | M | Teat | >16 | 0.047 | ≤0.5 | 0.094 | TEM-1 | F | APR CIP, NEO NAL STR SXT TET | |
| M9-35 | M | Milk | >16 | 0.032 | ≤0.5 | 0.064 | TEM-1 | ccdAB, vagCD, srnBC | F | NAL STR TET |
| M9-36 | P | Cattle feces | 8 | 0.032 | ≤0.5 | 0.064 | TEM-1 | hok-sok, pndAC, srnBC | F | NEO NAL STR SXT TET |
| M9-37 | P | Cattle feces | 8 | 0.032 | ≤0.5 | 0.064 | TEM-1 | hok-sok, pndAC, srnBC | F | APR CIP NEO NAL STR SXT TET |
| M9-41 | P | Teat | 4 | 0.023 | ≤0.5 | 0.064 | hok-sok, pndAC, srnBC | F, I1-Iγ | TET | |
| M9-42 | P | Teat | >16 | 0.023 | ≤0.5 | 0.064 | hok-sok, srnBC | F | ||
| M9-43 | P | Teat | 16 | 0.023 | ≤0.5 | 0.064 | hok-sok, srnBC | F | ||
| M9-44 | P | Teat | 16 | 0.032 | ≤0.5 | 0.064 | hok-sok, srnBC | F | ||
| M9-45 | P | Teat | 12 | 0.023 | ≤0.5 | 0.064 | hok-sok, srnBC | F | ||
a
Etest, Epsilometer test; NT, not typeable by PCR-based replicon typing; CT, cefotaxime; CTL, cefotaxime-clavulanic acid; TZ, ceftazidime; TZL, ceftazidime-clavulanic acid; APR, apramycin; CHL, chloramphenicol; CIP, ciprofloxacin; GEN, gentamicin; NEO, neomycin; NAL, nalidixic acid; STR, streptomycin; SXT, trimethoprim-sulfamethoxazole; TET, tetracycline.
b
Underlined bla genes, addiction system and resistance markers transferred to the recipient E. coli J53 strain by conjugation.