Table 3.
Characteristics of 26 conjugation-positive E. coli isolates carrying blaCTX-M-32 genes described in this studya
| Isolateb | Origin | Etest MIC (mg/liter) |
Other β-lactamase | Addiction system(s)c | Transconjugant plasmid Inc type(s) | Non-β-lactam resistance pattern | |||
|---|---|---|---|---|---|---|---|---|---|
| CT | CTL | TZ | TZL | ||||||
| M1-4 | Cattle feces | >16 | 0.023 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, N, P, I1-Iγ | CHL CIP GEN NEO NAL STR SXT TET |
| M1-5 | Cattle feces | >16 | 0.023 | 32 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | N | CHL CIP GEN NEO NAL STR SXT TET |
| M1-6 | Cattle feces | >16 | 0.023 | 16 | 0.094 | TEM-1 | hok-sok, srnBC | N | CHL CIP NEO NAL STR SXT TET |
| M1-7 | Cattle feces | >16 | 0.023 | 32 | 0.023 | relBE | N | CHL CIP NEO NAL STR SXT TET | |
| M1-8 | Cattle feces | >16 | 0.023 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N | CHL CIP GEN NEO NAL STR SXT TET |
| M1-9 | Calf feces | >16 | 0.032 | 16 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | P, I1-Iγ | AMC CIP NEO NAL STR SXT TET |
| M1-11 | Manure | >16 | 0.023 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-12 | Ventilation | >16 | 0.023 | 12 | 0.125 | TEM-1 | hok-sok, srnBC | F, FIB, N | CHL CIP NEO NAL STR SXT TET |
| M1-13 | Ground floor | >16 | 0.023 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-14 | Ground fence | >16 | 0.016 | 8 | 0.094 | TEM-1 | hok-sok, srnBC | N | CHL CIP NEO NAL STR SXT TET |
| M1-15 | Parlor floor | >16 | 0.016 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-16 | Parlor fence | >16 | 0.016 | 12 | 0.094 | TEM-1 | pndAC | N, P, I1-Iγ | CHL CIP GEN NEO NAL STR SXT TET |
| M1-17 | Teat | >16 | 0.023 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N | CHL CIP NEO NAL STR SXT TET |
| M1-18 | Teat | >16 | 0.023 | 8 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-19 | Teat | >16 | 0.023 | 12 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | N | CHL CIP GEN NEO NAL STR SXT TET |
| M1-20 | Teat | >16 | 0.023 | 8 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | NT | CHL CIP NEO NAL STR SXT TET |
| M1-21 | Teat | >16 | 0.023 | 12 | 0.125 | TEM-1 | hok-sok, srnBC | F, FIB, N | AMC CHL CIP NEO NAL STR SXT TE |
| M1-22 | Teat | >16 | 0.023 | 8 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-23 | Teat | >16 | 0.016 | 12 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-24 | Milk | >16 | 0.032 | 16 | 0.032 | TEM-1 | hok-sok, pndAC, srnBC | FIB, N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-25 | Milk | >16 | 0.023 | 32 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | FIB, N, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-26 | Milk | >16 | 0.032 | 8 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | N | CHL CIP NEO NAL STR SXT TET |
| M1-27 | Milk | >16 | 0.023 | 8 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, P, I1-Iγ | CHL CIP GEN NEO NAL STR SXT TET |
| M1-28 | Milk | >16 | 0.023 | 12 | 0.125 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, N, P, I1-Iγ | APR CHL CIP NEO NAL STR SXT TET |
| M1-29 | Milk | >16 | 0.023 | 12 | 0.125 | TEM-1 | hok-sok, srnBC | F, FIB, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
| M1-30 | Milk | >16 | 0.023 | 8 | 0.094 | TEM-1 | hok-sok, pndAC, srnBC | F, FIB, P, I1-Iγ | CHL CIP NEO NAL STR SXT TET |
a
Etest, Epsilometer test; NT, not typeable by PCR-based replicon typing; CT, cefotaxime; CTL, cefotaxime-clavulanic acid; TZ, ceftazidime; TZL, ceftazidime-clavulanic acid; AMC, ampicillin-clavulanic acid; APR, apramycin; CHL, chloramphenicol; CIP, ciprofloxacin; GEN, gentamicin; NEO, neomycin; NAL, nalidixic acid; STR, streptomycin; SXT, trimethoprim-sulfamethoxazole; TET, tetracycline.
b
All E. coli strains carrying blaCTX-M-32 genes were isolated from farm N.
c
Underlined bla genes, addiction systems and resistance markers transferred to the recipient E. coli J53 strain by conjugation.