Table 1.
Site-directed mutagenesis studies on the lanthipeptides epidermin and gallidermin
| Epidermin/gallidermin mutation | Effect of mutationa | Reference(s) |
|---|---|---|
| Ser3Asn | Elimination of activity | 26 |
| Leu6Val | 5-fold reduction in activity against S. aureus Cowan I | 26 |
| Leu6Gly | >7-fold reduction in activity against S. aureus Cowan I | 26 |
| Gly10Glu | Elimination of activity | 26 |
| Ala12Leu | Loss of pore formation; 3-fold reduction in activity against S. aureus Cowan I | 25, 26 |
| Dhb14Dha | No change in activity against S. aureus Cowan I | 26 |
| Dhb14S | 6-fold reduction in activity against S. aureus Cowan I | 26 |
| Dhb14A | >7-fold reduction in activity against S. aureus Cowan I | 26 |
| Dhb14P | >7-fold reduction in activity against S. aureus Cowan I | 26 |
| Ser16Thr | Elimination of activity | 26 |
| Ser19Ala | Elimination of activity | 26 |
| Ser19Thr | Elimination of activity | 26 |
| Tyr20Gly | Elimination of activity | 26 |
| ΔCys21 | Elimination of activity | 26 |
| ΔCys22 | Elimination of activity | 26 |
a
The general summary of the effect of each mutant is provided relative to its activity.