Fig 3.
The results of batch experiments. (A to C) Influence of temperature, pH, and salinity on anammox activity determined by the 29N2 gas production rate. (D) Incorporation of [14C]bicarbonate and ammonium consumption by anammox bacteria. Incorporation of [14C]bicarbonate (vertical axis) increased with increases in the ammonium consumption rate (horizontal axis). Filled circles represent the value of “Candidatus Scalindua sp.” at 28°C, whereas an open circle represents the value at 37°C. Filled and open squares represent the values of “Candidatus Brocadia sinica” at 28 and 37°C, respectively. Biomass yields, 0.030 and 0.063 mol C (mol NH4+)−1, were obtained by dividing the amount of incorporated [14C]bicarbonate by the amounts of consumed ammonium obtained under different temperature conditions. All values are means ± standard deviations of the results of independent triplicate experiments.