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. 2013 Jul;79(13):3917–3925. doi: 10.1128/AEM.00714-13

Fig 2.

Fig 2

Western blot analysis of affinity-purified alga-produced CtxB-Pfs25. (A) Affinity-purified Cr.Pfs25 and Cr.CtxB-Pfs25 were reduced, separated by SDS-PAGE, transferred to nitrocellulose, and detected with anti-FLAG MAbs. (B and C) Reduced and nonreduced Cr.CtxB-Pfs25 were resolved by SDS-PAGE, transferred to nitrocellulose, and detected with anti-Pfs25 4B7 MAbs (B) and anti-cholera toxin Abs (C). (D) Five micrograms of nonreduced and reduced Cr.CtxB-Pfs25 and a BSA standard (2.5 μg, 1.25 μg, 1.125 μg, 0.56 μg) were resolved by SDS-PAGE (4 to 20% gradient) and stained with Coomassie blue. R, reduced; N, nonreduced.