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GM1 ganglioside binding by alga-produced CtxB-Pfs25. Soluble protein extracts (20 μg/ml) from JAG101 and ΔpsbA C. reinhardtii strains collected at 0, 24, 48, and 72 h after light induction (JAG101 is driven by the light-dependent psbA promoter) were applied to GM1 ganglioside-coated ELISA plates in triplicate. Binding was detected with anti-CT antibodies. Error bars shown are 1 standard deviation.
