Fig 5.

Immunogenicity of alga-produced CtxB-Pfs25 and Pfs5 using Alhydrogel. Mice were vaccinated by intraperitoneal injection with 20 μg of affinity-purified Cr.CtxB-Pfs25 or the molar equivalent of Cr.Pfs25 adsorbed to Alhydrogel. Pfs25-specific IgG antibodies were detected by ELISA. Endpoint titers were defined as the reciprocal of the last dilution that gave an absorbance greater than prebleed sera plus 3 standard deviations. Comparisons between groups were done using a two-tailed parametric Student t test (*, P < 0.05; **, P < 0.01).