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. 2013 Jul;79(13):4115–4128. doi: 10.1128/AEM.00817-13

Table 1.

The cp32 DNA elements and associated erp loci naturally contained in the analyzed Lyme disease-causing spirochetesa

Genospecies and strain cp32 segregation locus type
cp32-1 cp32-2/7 cp32-3 cp32-4 cp32-5 cp32-6 cp32-8 cp32-9 cp32-10 cp32-11 cp32-12 cp32-13
B. burgdorferi B31b erpA1B2 erpCD erpG erpHY erpAB5 erpK erpAB8 erpPQ erpX
erpLM
B. burgdorferi BL206b erpLM erpG erpHY erpAB5 erpK erpPQ erpX erpAB11
B. burgdorferi bu Bol26 R10 V38 N41, N42 Q34 W10 X41, X42
B. burgdorferi 297c ospE1, elpB1-1 elpA2 ospF elpA1 ospE5, elpB1-5 bbk2.10 p21, elpB2 bbk2.11 ospE12, elpB1-12
B. burgdorferi Sh-2-82 erp41-1, erp42-2 erp43 erp44 erp45 erp41-5, erp42-5 erp46 erp50, erp51 erp47, erp48 erp49 erp41-12, erp42-12
B. burgdorferi 156a P15 O27 S39 R40 M38 L38, L39 W37 X39, X40
B. burgdorferi ZS7d P41 AC26, AC64 R38, R39 N42, N43 AC26, AC64 X38
B. burgdorferi 64be P38 O38 SL81, SL82 R39, R40 V38, V39 M38, M39 SL81, SL82 Truncated plasmid, no erp locus W38
B. burgdorferi WI91-23 P39 O42 Truncated plasmid, no erp locus V22 M39, M40 Q39 W40, W41
B. burgdorferi 94a O39 V39 M40, M41 Q40, Q41 W38, W39
B. burgdorferi JD1f PV38, PV80 S38 M38, M39 L38, L39 N44, N45 Q40 W44 X44
B. burgdorferi CA-11.2A P40 S38 V38 Q39, Q40 N38
B. burgdorferi 118ag ON39, ON83 R38 V25 M39, M40 L38, L39 ON39, ON83 Q40, Q41 AB39
B. burgdorferi N40 ospEF erp23, erp24 erp25 p21, erp22 erp26 erp27
B. burgdorferi 72a O40 Truncated plasmid, no erp locus V24 Q39, Q40 X36 N39
B. burgdorferi 29805 O38 R39, R40 V28 N43, N44 X36
SV1h (unnamed species) O39, O40 S38, S39 Truncated plasmid, no erp locus M31 X50, X51
B. valaisiana VS116 O43 (aka H460) V36, V37 Q36
B. bissettii DN127i O40 S39 R38, R39 V40 M40 (i) (i) (i)
B. afzelii PKo P38 O31 S39 V39 N36, N37 W37 X38, X39
B. afzelii ACA-1 P41, P42 S38, S39 R38 V40
B. garinii PBr V40 Q67
a

Plasmid nomenclature is standardized according to segregation locus type, such that all plasmids designated cp32-1, for example, contain a similar segregation locus. All cp32 segregation loci fell into the 12 previously described groups (see Fig. 1). Note that the first examined strain, B. burgdorferi B31, contains 2 distinct prophages that possess identical maintenance loci, cp32-2 and cp32-7, and define segregation locus type 32-2/7. As a result, the numbering scheme for the 12 groups goes up to cp32-13. For those erp genes that have been previously described, the published names are used. Various naming schemes were applied to these genes by their discoverers. Previously undescribed erp alleles found by mining GenBank are identified by the open reading frame designations of the entries.

b

The cp32-10 plasmids of strain B31 and BL206 are naturally integrated into an unrelated linear replicon, which created the ca. 56-kb linear plasmid lp56.

c

The cp32-7 and cp32-9 plasmids of strain 297 are naturally truncated and have been named cp18-a and cp18-2, respectively.

d

The open reading frames are designated with the two letters, AC.

e

The cp32-3 and cp32-8 plasmids of strain 64b are fused together into a single circular replicon, cp32-3-8. Its open reading frames are designated with the two letters, SL.

f

The cp32-1 and cp32-5 of strain JD1 are fused together into a single circular replicon, cp32-1-5. Its open reading frames are designated with the two letters, PV.

g

The cp32-2/7 and cp32-9 plasmids of strain 118a are fused into a single circular replicon, cp32-7-9. Its open reading frames are designated with the two letters, ON.

h

Strain SV1 also contains an additional legitimate erp locus (ORF A100) on a linear plasmid that contains an lp54-type replication locus. This strain falls within an as-yet-unnamed group of Lyme disease-causing Borrelia.

i

Strain DN127 contains a large circular plasmid that includes portions of four distinct cp32s and includes replication loci of types cp32-9, cp32-11, and cp32-12. Three erp loci are located on this chimera, two monocistronic and one bicistronic. For the purposes of this study, the erp genes are designated open reading frames Quad-A, Quad-B1, Quad-B2, and Quad-C.