Fig 4.

Effect of MβCD on L. infantum chagasi metacyclic promastigote attachment to and survival in macrophages. Human MDMs were infected at an MOI of 5 with opsonized metacyclic promastigotes of untreated control or MβCD-treated (25 mM, 1 h) groups in the absence (−) or presence (+) of cytochalasin D (Cyto) and fixed 30 min later to assess attachment or 24 and 72 h later to assess survival. Slides stained with Wright-Giemsa stain demonstrate a lack of internalized parasites in the presence of cytochalasin D. Scale bar, 20 μM.