Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant genotype or characteristicsa | Reference |
|---|---|---|
| E. coli | Invitrogen | |
| DH5α | F− ϕ80ΔlacZM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17(rK− mK−) | |
| S17-1λpir | Tpr Strr recA thi pro hsdR hsdM+ RP4::2-Tc::Mu::Km Tn7; λ pir lysogen | 44 |
| Y. enterocolitica | ||
| JB580v | 8081v (r− m+ Nalr), serotype O:8 | 45 |
| JB580c | JB580v cured of pYVe8081 | Lab strain |
| YVM1178 | JB580v ΔysaC | 31 |
| YVM1184 | JB580c ΔysaC | This work |
| YVM1374 | JB580v ΔyscC | 31 |
| YVM1629 | JB880v ΔysaC ΔyscC | This work |
| YVM1600 | JB580c ΔyspD | This work |
| YVM1370 | JB580v Δinv | This work |
| YVM1390 | JB580c Δinv | This work |
| YVM1520 | JB580c ΔyspA | This work |
| YVM1521 | JB580c ΔyspE | This work |
| YVM1522 | JB580c ΔyspF | This work |
| YVM1523 | JB580c ΔyspI | This work |
| YVM1524 | JB580c ΔyspK | This work |
| YVM1525 | JB580c ΔyspL | This work |
| YVM1528 | JB580c ΔyspM | This work |
| YVM1526 | JB580c ΔyspP | This work |
| YVM1527 | JB580c ΔyspY | This work |
| YVM11511 | JB580c ΔyspAF | This work |
| YVM1550 | JB580c Δysp9 (ΔyspAEFIKLMPY) | This work |
| Plasmids | ||
| pSR47S | Kanr; MobRP4 oriR6K, cloning vector | 24 |
| pGEN-lux | Ampr; source of em7 promoter for pJH026 | 22 |
| pPROBE-gfp[tagless] | Kanr; gfp transcriptional fusion plasmid | 23 |
| pMWO-073 | Specr; low-copy-number expression vector | 25 |
| pJH026 | Kanr; em7 promoter cloned into pPROBE-gfp[tagless] | This work |
| pKW60 | Kanr; yspA in-frame deletion (codons 6–637 deleted) | This work |
| pKW54 | Kanr; yspE in-frame deletion (codons 11–380 deleted) | This work |
| pKW59 | Kanr; yspF in-frame deletion (codons 5–346 deleted) | This work |
| pKW12 | Kanr; yspI in-frame deletion (codons 29–137 deleted) | This work |
| pKW91 | Kanr; yspK in-frame deletion (codons 12–176 deleted) | This work |
| pKW56 | Kanr; yspL in-frame deletion (codons 2–700 deleted) | This work |
| pBF001 | Kanr; yspM in-frame deletion (codons 14–275 deleted) | This work |
| pKW57 | Kanr; yspP in-frame deletion (codons 8–382 deleted) | This work |
| pKW55 | Kanr; yspY in-frame deletion (codons 13–444 deleted) | This work |
| pKW106 | Kanr; yspD in-frame deletion (codons 20–319 deleted) | This work |
| pEll26 | Kanr; inv in-frame deletion (codons 4–833 deleted) | This work |
| pKW132 | Specr; ysaC coding region in pMWO-073 | This work |
| pKW133 | Specr; yspD coding region in pMWO-073 | This work |
a
Kan, kanamycin; Amp, ampicillin; Spec, spectinomycin.